1064 The effect of low energy laser irradiation on the proliferation of cells in culture
Study Overview
- Description: This study investigated the effects of Low Energy Laser Irradiation (LELI), or Low-Level Laser Therapy (LLLT), on the proliferation of skin fibroblasts, muscle cells, and lymphocytes grown in vitro.
- Source: PubMed
Summary
- Background: LELI has been widely used to accelerate wound healing, reduce inflammation, relieve pain, and treat various musculoskeletal conditions. These effects are claimed to be mediated by stimulating cell proliferation, metabolism, and the release of various active factors.
- Methods: The researchers used a Helium-Neon (HeNe) laser (wavelength 632.8 nm) to irradiate three different types of in vitro cultured cells:Skin fibroblastsMuscle cells (myoblasts)Lymphocytes
- Skin fibroblasts
- Muscle cells (myoblasts)
- Lymphocytes
- Results:Dose-Dependent Effect: The effect of LELI on cell proliferation was highly dependent on the dose applied.Low Dose Effect: At low doses of irradiation, the laser demonstrated a stimulatory effect on the proliferation of all three cell types.High Dose Effect: At high doses of irradiation, the laser exhibited an inhibitory effect on cell proliferation.Biphasic Effect: This phenomenon is known as the biphasic dose response, which is consistent with the Arndt-Schultz Law (though the law itself is not explicitly mentioned in the abstract, the effect described is the same: low dose stimulates, high dose inhibits).
- Dose-Dependent Effect: The effect of LELI on cell proliferation was highly dependent on the dose applied.
- Low Dose Effect: At low doses of irradiation, the laser demonstrated a stimulatory effect on the proliferation of all three cell types.
- High Dose Effect: At high doses of irradiation, the laser exhibited an inhibitory effect on cell proliferation.
- Biphasic Effect: This phenomenon is known as the biphasic dose response, which is consistent with the Arndt-Schultz Law (though the law itself is not explicitly mentioned in the abstract, the effect described is the same: low dose stimulates, high dose inhibits).
- Conclusion: Low energy laser irradiation has a biphasic effect on cell proliferation. This finding underscores the critical importance of selecting the optimal therapeutic dose when using LELI clinically to ensure the desired biostimulatory effects are achieved, rather than inhibition.
